snp genotyping by sequenom massarray maldi-tof mass spectrometry  (Sequenom)

Journal: PLOS ONE

Article Title: Altered GC- and AT-biased genotypes of Ophiocordyceps sinensis in the stromal fertile portions and ascospores of natural Cordyceps sinensis

doi: 10.1371/journal.pone.0286865

Figure Lengend Snippet: GT refers to genotype. Genotypes #1‒2 and #13‒14 are GC-biased O . sinensis genotypes, and Genotypes #4‒6 and #15‒17 are AT-biased O . sinensis genotypes. The GC and AT contents of the 17 O . sinensis genotypes are shown in . The underlined sequences in black represent primers for the first-step PCR. Extension primers 067721–477, 067721–531, 067740–324, 067744–324 (RC, reverse complement) and 067740–328 (RC) in brown were used for SNP mass spectrometry genotyping to distinguish between genotypes of O . sinensis . Arrows indicate the directions of the primer extension reactions toward the SNP alleles in brown. “-” refers to an identical base, and spaces refer to unmatched sequence gaps.

Article Snippet: Reagents and biochips required for MassARRAY SNP MALDI-TOF MS genotyping were from Sequenom, USA (acquired by Agena Bioscience Company).

Techniques: Mass Spectrometry, Sequencing

Journal: PLOS ONE

Article Title: Altered GC- and AT-biased genotypes of Ophiocordyceps sinensis in the stromal fertile portions and ascospores of natural Cordyceps sinensis

doi: 10.1371/journal.pone.0286865

Figure Lengend Snippet: SNP extension primers for MassARRAY MALDI-TOF MS genotyping.

Article Snippet: Reagents and biochips required for MassARRAY SNP MALDI-TOF MS genotyping were from Sequenom, USA (acquired by Agena Bioscience Company).

Techniques: Sequencing

Journal: PLOS ONE

Article Title: Altered GC- and AT-biased genotypes of Ophiocordyceps sinensis in the stromal fertile portions and ascospores of natural Cordyceps sinensis

doi: 10.1371/journal.pone.0286865

Figure Lengend Snippet: GT refers to genotype. Genotypes #1‒2 and #13‒14 are GC-biased O . sinensis genotypes, and Genotypes #4‒6 and #15‒17 are AT-biased O . sinensis genotypes. The GC and AT contents of the 17 O . sinensis genotypes are shown in . The underlined sequences in black represent primers for the first-step PCR. Extension primers 067721–477, 067721–531, 067740–324, 067744–324 (RC, reverse complement) and 067740–328 (RC) in brown were used for SNP mass spectrometry genotyping to distinguish between genotypes of O . sinensis . Arrows indicate the directions of the primer extension reactions toward the SNP alleles in brown. “-” refers to an identical base, and spaces refer to unmatched sequence gaps.

Article Snippet: The MassARRAY SNP MALDI-TOF MS genotyping experiments were performed by a service company, CapitalBio Co., in Beijing, China.

Techniques: Mass Spectrometry, Sequencing

Journal: PLOS ONE

Article Title: Altered GC- and AT-biased genotypes of Ophiocordyceps sinensis in the stromal fertile portions and ascospores of natural Cordyceps sinensis

doi: 10.1371/journal.pone.0286865

Figure Lengend Snippet: SNP extension primers for MassARRAY MALDI-TOF MS genotyping.

Article Snippet: The MassARRAY SNP MALDI-TOF MS genotyping experiments were performed by a service company, CapitalBio Co., in Beijing, China.

Techniques: Sequencing